quantitative determination of protein

Please follow the following guideline in submitting your Lab 6. Estimate the protein from the standard graph and provide this information in the discussion part of your report. If your report does not meet these requirements it will be graded.

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Lab Reports


A description of the main idea or question of the lab. This can also highlight a key finding or question.


A brief summary of the main question, methods and findings. This is usually the last thing written but

the first thing presented in order to grab the attention of the reader.

A rough breakdown of an abstract would contain about:

• 3 sentences worth of introduction with the key question

• 2 sentences of major methodology

• 3-6 sentences of the major results and conclusions drawn from them

Lengths will vary, but using this framework, you will not deviate too far from having a reader lose



Introduce the background that is relevant for forming the hypotheses being tested. What were the

previous observations or prior knowledge used to come to these ideas? State the actual hypotheses

to be tested and how it will further the understanding of the issue.

Materials and Methods

This section is a little like a cooking recipe. The main steps taken should be summarized as a standard

prose in a manner that anyone could follow and repeat. This is written in the past tense and 3rd

person. Do not write in the first person as *you* have nothing to do with the experiments. Explain

“What was done with which reagents?”


This section is descriptive of what was observed. Figures and tables serve as a summary of the results

to illustrate the data. They also serve as guides to outline the text of the section. Slowly describe each

figure or table or graphs. Expand these points into sentences and paragraphs. Present the data as fully as possible, including stuff that at the moment does not quite make sense. This is written in the past

tense and 3rd person. Conclusions are not provided in this section as they are made from analyzing

the information and synthesizing the results.


Discussions are the conclusions made through analyzing the results. At this time, you will be able to

re-emphasize the original hypotheses made in the introduction. Indicate whether or not the

hypotheses were demonstrated sufficiently. If this is not the case, offer alternatives and

interpretations. Can you improve or modify your hypotheses? Explain how multiple lines of evidence

corroborate each other and help to further the understanding of the problem.

Reporting on Science


Prior knowledge requires demonstration of strength or validity. The references should be from

primary sources and should illustrate the point of the statement. The section is presented in

numerous formats as a bibliography, but citations are inserted near the text where knowledge or

statements are displayed.

Use the data below to determine the concentration
of protein in unknown samples

Protein Concetration(mg/ml) Absorbance at 540 nm
0 0
1 0.06
2 0.125
4 0.25
6 0.37
8 0.49
10 0.62

Unknown-1 ? 0.13
Unknown-2 ? 0.4
Unknown-3 ? 0.5

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